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Tagging of Adult House Flies and Flesh Flies with Radioactive Phosphorus¹

A method has been developed for radioactively tagging adult M. domestica, C. macellaria and P. pallescens with P³² incorporated into milk fed to the adult flies. Low activity was shown by adult flies from larvae reared in media containing P³².

The females attained higher levels of initial activity and retained more P³² than did the males in all species. Different levels of activity were shown by the various species. Adult flies from 1 to 6 days old showed only minor differences in initial uptake of P³² with a one-day feeding period. The initial uptake of P³² was approximately proportional to the concentration of P³² in the various milk solutions within the range tested.

Decline in radioactivity was influenced by different diets of the adult flies after the P³² feeding and may be correlated with the phosphorus content of these diets.

Practical tests have shown that tagged flies can be detected in trap catches or on bait stations. Determinations of radioactivity of the flies in trap catches is the more efficient method.

¹ From the Communicable Disease Center, Public Health Service, Federal Security Agency, Atlanta, Georgia. Technical Development Services, Savannah, Ga.

² Sanitary Engineer (R).

³ Entomologist.