HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nakamura, M. Articles by Anderson, H. H. Search for Related Content PubMed Articles by Nakamura, M. Articles by Anderson, H. H.

Effect of Heat-Treatment on the Respiration of Trypanosoma Cruzi Used for the Cultivation of Endamoeba Histolytica

1. E. histolytica heat-treated at 45°C., 48°C., and 50°C. for 10 minutes survived when transplanted onto unheated T. cruzi or trypanosomes heated at 45°C. and 48°C. for 10 minutes, but failed to survive when associated with trypanosomes heat-treated at 50°C.

2. Subcultures of T. cruzi after heating at 48°C. and 50°C. failed to produce growth.

3. Oxygen uptake studies indicated that there is a pronounced respiration with 1 ml. of T. cruzi with a count of 100,000,000 per ml. heat-treated at 48°C. for 10 minutes, but no respiration occurs with T. cruzi heat-treated at 50°C. for 10 minutes.

4. The efficacy of T. cruzi associates in the cultivation of E. histolytica in vitro was demonstrated after heat-treatment that rendered the trypanosomes non-viable but did not extend to temperatures that inhibited their respiration.

¹ With the assistance of Miss D. Swanman and Mrs. Betty Mythen.

² Supported in part by the National Institutes of Health, Bethesda 14, Md., and the Abbott Laboratories, North Chicago, Illinois.