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Persistence of Yellow Fever Virus in Mosquitoes after Death of the Insect¹

Significant amounts of yellow fever virus have been recovered from the bodies of dead Aedes aegypti as long as 4 hours after death of the insect from exposure to ether, 17.5 hours after death from starvation, 20 hours after death from exposure to tobacco smoke, and 45.5 hours after death from exposure to potassium cyanide fumes.

On the contrary, following death from exposure to chloroform vapor, most of the virus activity was lost in 30 minutes and was totally absent in 1 hour.

Virus has been recovered from a significant number of lots of Haemagogus equinus and Haemagogus spegassinii which died of "natural causes" during the course of laboratory transmission experiments.

Received December 16, 1944.
¹ The work on which these observations are based was carried out under the auspices of the Serviço de Estudos e Pesquisas sôbre a Febre Amarela (Yellow Fever Research Service), which is maintained jointly by the Ministry of Education and Health of Brazil and the International Health Division of The Rockefeller Foundation.