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A Method for the Collection, Transportation and Study of Anopheline Eggs and Adults^*

That the accurate and adequate study of mosquito eggs in conjunction with all stages of the life cycle is important has become a well-recognized tenet concerning the conduct of anopheline surveys. One of the chief problems, however, associated with a survey covering an extensive area is that of getting viable eggs to the research laboratory. During an anopheline survey in northeastern Brazil a simple and satisfactory method was worked out for the collection and shipment of eggs and mosquitoes. A brief description of the method is herewith given.

Females were captured by inspectors in dwelling houses and on animal bait out of doors with the Godoy suction tube, and transported in screen cages to their local quarters. Here they were isolated singly in small cotton-stoppered, flat-bottomed vials 2 x 5 cms., containing about 2 cc. of water (Plate I, fig. 1). Special care was taken not to wet the specimen during the process of isolation.

^* The studies herewith reported were part of the program of the Serviço de Malaria do Nordeste maintained by the Ministry of Education and Health of Brazil, and the International Health Division of The Rockefeller Foundation.