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The Use of Nutrient Agar for Rearing Dipterous Larvae

A study of the biology of Tabanidae was begun during the past summer, at the suggestion and with the help of Dr. Robert Matheson, in which the writer made use of the ordinary nutrient agar to provide a suitable medium for the larvae.

The writer learned of the use of agar for this purpose from Dr. J. Chester Bradley's journal which he sent to the college while on his collecting trip to South America. Dr. Bradley wrote:

Dr. Luts has a remarkably interesting method of rearing the larvae of Tabanidae and other mud-dwelling species. He puts them into a glass dish and covers them with agar, which forms a transparent substitute for mud which pleases them very well. They lie perfectly still in this for days at a time and at intervals he feeds them by putting in a snail or two, or better still, when he has them some oligochaete worms.

Received May 27, 1922.