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The Resistance of Endamoeba Histolytica Cysts to Chlorine in Aqueous Solutions

In reviewing the literature there seems to be a unanimous opinion that the cysts of Endamoeba histolytica are much more resistant to the action of chlorine in aqueous solutions than the ordinary vegetative bacteria. Because of this opinion, backed by the experimental work of numerous able investigators, it was felt by the author that chlorine might be used to free cultures of E. histolytica of the accompanying bacterial flora, if none of the spore bearing organisms were present. An unlimited supply of cysts of E. histolytica obtained by the cultural method (1) was available and the bacterial flora present contained no spore bearing organisms. Utilizing this material, various concentrations of chlorine in sterile tap water were used in an attempt to obtain viable cysts free from bacteria. It was found that when a sufficient concentration of chlorine was used to kill the bacteria (Escherichia coli and Streptococcus fecalis) present, the cysts were also killed.

Received May 25, 1937.