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Morphological Differentiation of Entamoeba Hartmanni and E. Polecki from E. Histolytica^*

1. Hematoxylin staining is the most accurate technic for differentiating E. hartmanni and E. polecki from E. histolytica.

2. Entamoeba hartmanni may be distinguished from the dwarf forms of E. histolytica by the smaller sizes of the trophozoites; by the smaller and more E. coli-type of nucleus; by the smaller sizes of the cysts; by the smaller percentage of nuclear diameter to cyst diameter; and by the diffuse type of cyst vacuolation.

3. Entamoeba polecki may be distinguished from the larger E. histolytica by the more variable nuclear structure of trophozoites and cysts; by the frequently darker nucleoplasm; by the predominately uninuclear type of cyst; by the smaller percentage which nuclear diameter is of cyst diameter; by cytoplasmic condensations and inclusion masses; and by the diffuse type of cyst vacuolation.

^* Presented before the New York Society of Tropical Medicine on 26 March 1959.