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Am. J. Trop. Med. Hyg., 79(1), 2008, pp. 28-35
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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Differential Tissue and Flight Developmental Expression of Glycerol-3-Phosphate Dehydrogenase Isozymes in the Chagas Disease Vector Triatoma infestans

María Mercedes Stroppa*, Carlota Carriazo, Néstor Soria, Rodolfo Pereira, AND Nelia M. Gerez de Burgos
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Nacional de Córdoba, Córdoba, Argentina

Glycerol-3-phosphate dehydrogenase (GPDH) isozymes are differentially expressed among tissues and during flight development. GPDH-1 is involved in the flight-muscle metabolism and GPDH-2 provides precursors for lipid biosynthesis in many tissues. We have isolated and characterized from Triatoma infestans, a Chagas disease vector, two cDNAs encoding for GPDH-1 and GPDH-2 isozymes. The inferred amino acid sequences showed high identity with other GPDH sequences from flying insects. A GPDH-2 transcript was found in fifth instar nymphs, thoracic muscles, adult gonads, and fat bodies. Both isozymes are present in 30-day-old adult thoracic muscle transcripts, and the pattern of expression differs between sexes. The expression of GPDH-1 begins earlier in females, and GPDH-2 is expressed more abundantly in female adult thoracic muscles than in those from males. This finding is consistent with those of other investigators who showed a higher flight initiation probability in T. infestans females than in males.

Received November 23, 2007. Accepted for publication April 1, 2008.

Acknowledgments: We thank Antonio Blanco for helpful suggestions and critical revision of the manuscript, and the Insectary of the Reference Center of Vectors, National Service of Chagas, Córdoba, Argentina, for providing insects used in our studies.

Financial support: This study was supported in part by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina) and Secretaría de Ciencia y Tecnología (SECYT) de la Universidad Nacional de Córdoba, Argentina. Néstor Soria, Rodolfo Pereira is a Career Member of CONICET and Mara í Mercedes Stroppa is a fellow of CONICET.

* Address correspondence to María Mercedes Stroppa, Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Pabellón Argentina, 2 do Piso, Ciudad Universitaria, Córdoba, CP 5000, Argentina. E-mail: mercedesstroppa{at}hotmail.com

Authors’ addresses: María Mercedes Stroppa, Carlota Carriazo, Néstor Soria, Rodolfo Pereira, and Nelia M. Gerez de Burgos, Departamento de Bioquímica y Biologìa Molecular, Facultad de Ciencias Médicas, Pabellón Argentina, 2 do Piso, Ciudad Universitaria, Córdoba, CP 5000, Argentina, Tel: 54-351-433-3024, Fax: 54-351-4333072, E-mail: mercedesstroppa{at}hotmail.com.






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Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.