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Am. J. Trop. Med. Hyg., 78(1), 2008, pp. 117-121
Copyright © 2008 by The American Society of Tropical Medicine and Hygiene

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Development of a Rapid Immunochromatographic Test for Simultaneous Serodiagnosis of Bovine Babesioses Caused by Babesia bovis and Babesia bigemina

Chul-Min Kim, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, AND Ikuo Igarashi*
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

With the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (BoiICT) was constructed for the simultaneous detection of Babesia bovis- and Babesia bigemina-specific antibodies using B. bovis recombinant merozoite surface antigen-2c and B. bigemina recombinant rhoptry-associated protein-1. The BoiICT selectively detected specific antibodies to B. bovis and B. bigemina. All sera from cattle infected with other protozoan parasites (i.e., Cryptosporidium parvum, Neospora caninum, and Theileria orientalis) showed negative results in the BoiICT. The relative sensitivity and specificity for detecting antibody to B. bovis were 96.7% (29 of 30) and 91.3% (73 of 80), respectively. The relative sensitivity and specificity for detecting antibody to B. bigemina were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment.

Received July 14, 2007. Accepted for publication October 4, 2007.

Acknowledgments: We thank Yoshio Nakamura (National Institute of Animal Heath) for providing sequential bovine sera of cattle experimentally infected with B. bovis and B. bigemina.

Financial support: This study was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, and grants from the Program for the Promotion of Basic Research Activities for Innovative Biosciences and the 21st Century COE Program (A-1), Ministry of Education, Culture, Sports, Science, and Technology, Japan.

* Address correspondence to Ikuo Igarashi, Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. E-mail: igarcpmi{at}obihiro.ac.jp

Authors’ address: Chul-Min Kim, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, and Ikuo Igarashi, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.

Reprint requests: Ikuo Igarshi, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan, Telephone: 81-155-49-5641, Fax: 81-155-49-5643, E-mail: igarcpmi{at}obihiro.ac.jp.






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Copyright © 2008 by the American Society of Tropical Medicine and Hygiene.