AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 77(5), 2007, pp. 812-813
Copyright © 2007 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Quantitation of B. Pseudomallei in Clinical Samples

Vanaporn Wuthiekanun, Direk Limmathurotsakul, Gumphol Wongsuvan, Wirongrong Chierakul, Nittaya Teerawattanasook, Prapit Teparrukkul, Nicholas P. Day, AND Sharon J. Peacock*
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Medical Department, Sappasithiprasong Hospital, Ubon Ratchathani, Thailand; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

 

ABSTRACT

We undertook a prospective study to quantitate Burkholderia pseudomallei in blood, pus, respiratory secretions, and urine obtained from 414 patients with melioidosis. The median was count 1.1, 1.5 x 104, 1.1 x 105, and 1.1 x 107 CFU/mL in these sample types, respectively. This provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material.

Received May 18, 2007. Accepted for publication July 19, 2007.

Acknowledgments: We are grateful for the assistance given by the staff of Sappasithiprasong and the Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program. Financial support: SJP was supported by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust.

* Address correspondence to Sharon J. Peacock, 420/6 Rajvithi Road, Bangkok 10400, Thailand. E-mail: Sharon{at}tropmedres.ac

Authors’ addresses: Vanaporn Wuthiekanun, Direk Limmathurot-sakul, Gumphol Wongsuvan, Wirongrong Chierakul, Nicholas P. J. Day, and Sharon J. Peacock, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. Nittaya Teerawattanasook and Prapit Teparrukkul, Sappasithiprasong Hospital, Ubon Ratchathani Province, Thailand.

Reprint requests: Sharon Peacock, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand, Telephone: 66-2-354-9172, Fax: 66-2-354-9169, E-mail: sharon{at}tropmedres.ac.






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Copyright © 2007 by the American Society of Tropical Medicine and Hygiene.