HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jacobson, J. A. Articles by Gibbons, R. V. Search for Related Content PubMed PubMed Citation Articles by Jacobson, J. A. Articles by Gibbons, R. V. Related Collections Diagnosis Fleas

Evaluation of Three Immunoglobulin M Antibody Capture Enzyme-linked Immunosorbent Assays for Diagnosis of Japanese Encephalitis

Japanese encephalitis (JE) virus is a major cause of neurologic infection in Asia, but surveillance has been limited. Three JE immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay kits have recently been developed. The aim of this study was to evaluate their sensitivity, specificity, and usability using 360 acute-phase serum samples containing JE, dengue, or neither IgM antibody. The kits, manufactured by Panbio Limited, Inbios International, Inc., and XCyton Diagnostics Ltd, had high sensitivities of 89.3%, 99.2%, and 96.7%, respectively. The specificities were 99.2%, 56.1%, and 65.3%, respectively. When dengue IgM-positive samples were excluded, the kits had specificities of 98.4%, 96.1%, and 96.1%, respectively. The Panbio kit includes both JE and dengue antigens and appears to have an advantage in settings where dengue virus co-circulates, although further assessments in clinical settings are needed. This information is helpful in considering options for strengthening the laboratory component of JE surveillance.

Received December 22, 2006. Accepted for publication April 3, 2007.

Acknowledgments: The authors thank Khun Nawarat Charoensri and Khun Panor Srisongkram, USAMC-AFRIMS, for their performance of the tests; Dr. Ananda Nisalak of USAMC-AFRIMS for technical advice; and Dr. Martha Roper for her contributions to the analyses of the results.

Financial support: This work was funded by the Bill & Melinda Gates Foundation.

^* Address correspondence to Julie A. Jacobson, Japanese Encephalitis Project, PATH, 1455 NW Leary Way, Seattle, Washington 98107. E-mail: jjacobs{at}path.org

Authors’ addresses: Julie A. Jacobson, Susan L. Hills, and Jennifer L. Winkler, Japanese Encephalitis Project, PATH, 1455 NW Leary Way, Seattle, WA 98107, Telephone: +1 (206) 285-3500, Fax: +1 (206) 285-6619, E-mails: jjacobs{at}path.org, shills{at}path.org, and jwinkler{at}path.org. Mammen Mammen, Butsaya Thaisomboonsuk, and Robert V. Gibbons, Department of Virology, United States Army Medical Component–Armed Forces Research Institute of Medical Sciences, 315/6 Rajvithi Road, Bangkok 10400, Thailand, Telephone: +662 644 5644, Fax: +662-644-4760, E-mails: mammen.mammen{at}us.army.mil, ButsayaT{at}afrims.org, and Robert.Gibbons{at}afrims.org. Anthony A. Marfin, Division of Global Migration and Quarantine, Centers for Disease Control and Prevention, 2201 Sixth Avenue, Seattle, WA 98121, Telephone: +1 (206) 615-3600, Fax: +1 (206) 615-2481, E-mail: aam0{at}cdc.gov,

Reprint requests: Julie Jacobson, Japanese Encephalitis Project, PATH, 1455 NW Leary Way, Seattle, WA 98107, Telephone: +1 (206) 285-3500, Fax: +1 (206) 285-6619, E-mail: jeproject{at}path.org.