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Am. J. Trop. Med. Hyg., 76(5), 2007, pp. 902-905
Copyright © 2007 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


PRODUCTION OF RECOMBINANT KINESIN-RELATED PROTEIN OF LEISHMANIA DONOVANI AND ITS APPLICATION IN THE SERODIAGNOSIS OF VISCERAL LEISHMANIASIS

HIDEKAZU TAKAGI*, MOHAMMAD ZAHIDUL ISLAM, MAKOTO ITOH, ANWAR UL ISLAM, A. R. M. SAIFUDDIN EKRAM, SULTANA MONIRA HUSSAIN, YOSHIHISA HASHIGUCHI, AND EISAKU KIMURA
Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh; Department of Medicine, Rajshahi Medical College, Rajshahi, Bangladesh; Encephalitis Surveillance in Bangladesh, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Japan

 

ABSTRACT

To detect IgG antibody in the serodiagnosis of visceral leishmaniasis (VL), a recombinant antigen rK39, which is part of a Leishmania chagasi kinesin-related protein, has been used successfully and showed high sensitivity and specificity. We report production of a recombinant protein rKRP42, which is part of an L. donovani kinesin-related protein and a homolog of rK39, and its application in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of VL. When rKRP42 and rK39 were compared, amino acid sequence analysis showed 89.3% identity and 98.7% homology, with rKRP42 having 39 more amino acids than rK39. The ELISA using rKRP42 showed a sensitivity of 94.6% (70 positive samples among 74 from VL patients) and a specificity of 99.3% (148 negative samples among 149 samples from Japanese controls), whereas the sensitivity of the commercial rK39 dipstick test was 93.2% (69 positive samples among 74 from patients with VL). The rKRP42 is a promising new antigen in developing immunodiagnostic methods for VL.

Received September 1, 2006. Accepted for publication January 18, 2007.

Financial support: This study was supported by Grant-in-Aid for Scientific Research for JSPS Postdoctoral Fellowship for Foreign Researchers no. 16. 04227, Grant-in-Aid for Scientific Research (B) no. 15406018, and Grant-in-Aid for Scientific Research (B) no. 18406013 from the Japan Society for the Promotion of Science.

* Address correspondence to Hidekazu Takagi, Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi-ken 480-1195, Japan. E-mail: htakagi{at}aichi-med-u.ac.jp

Authors’ addresses: Hidekazu Takagi, Mohammad Zahidul Islam, Makoto Itoh, and Eisaku Kimura, Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi-ken 480-1195, Japan, Telephone: 81-52-264-4811, Fax: 81-561-63-3645, E-mails: htakagi{at}aichi-med-u.ac.jp, zahid{at}aichi-med-u.ac.jp, macitoh{at}aichi-med-u.ac.jp, and kimura{at}aichi-med-u.ac.jp. Anwar Ul Islam, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh. Telephone: 88-721-750-0419, Fax: 88-721-750-064, E-mail: profanwarulislam{at}yahoo.com. A. R. M. Saifuddin Ekram, Department of Medicine, Rajshahi Medical College, Rajshahi, Bangladesh, Telephone: 88-721-776-0019, Fax: 88-721-772-174 E-mail: armsekram{at}yahoo.com. Sultana Monira Hussain, Encephalitis Surveillance in Bangladesh, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh, E-mail: smmuku{at}yahoo.com. Yoshihisa Hashiguchi, Department of Parasitology, Kochi Medical School, Kochi University, Nankoku City, Kochi 783-8505, Japan, Telephone: 81-88-880-2415, Fax: 81-88-880-2415, E-mail: hasiguti{at}med.kochi-ms.ac.jp.

Reprint requests: Hidekazu Takagi, Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi-ken 480-1195, Japan, Telephone: 81-52-264-4811, Fax: 81-561-63-3645, E-mail: htakagi{at}aichi-med-u.ac.jp.




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M. Z. Islam, M. Itoh, H. Takagi, A. U. Islam, A. R. M. S. Ekram, A. Rahman, A. Takesue, Y. Hashiguchi, and E. Kimura
Enzyme-linked Immunosorbent Assay to Detect Urinary Antibody Against Recombinant rKRP42 Antigen Made from Leishmania donovani for the Diagnosis of Visceral Leishmaniasis
Am J Trop Med Hyg, October 1, 2008; 79(4): 599 - 604.
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Copyright © 2007 by the American Society of Tropical Medicine and Hygiene.