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Am. J. Trop. Med. Hyg., 76(5), 2007, pp. 827-829
Copyright © 2007 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


EFFICIENT ORAL INFECTION OF CULEX PIPIENS QUINQUEFASCIATUS BY RIFT VALLEY FEVER VIRUS USING A COTTON STICK SUPPORT

SARA MOUTAILLER, MICHÈLE BOULOY, AND ANNA-BELLA FAILLOUX*
Unité Postulante Génétique Moléculaire des Bunyaviridés, Institut Pasteur, Paris, France

 

ABSTRACT

Transmission experiments are essential to assess vector competence. In an attempt to study Rift Valley fever virus (RVFV) transmission in its arthropod vector, disseminated infection rates were measured in Culex pipiens quinquefasciatus exposed to Clone 13, an avirulent variant of RVFV. We compared the classic system of glass feeders covered with different membranes (0–1%) with an alternative system in which a cotton stick is used as a support of a blood meal. The latter system showed the highest successful feeding rate (80%). Using this system, we optimized two parameters to obtain the highest numbers of engorged females and thus the highest numbers of females with disseminated infection: 1) use of freshly collected washed erythrocytes and 2) duration of the extrinsic incubation period of at least 14 days after infection.

Received July 3, 2006. Accepted for publication December 26, 2006.

Acknowledgments: We thank D. Coudrier and C. Tamietti for technical assistance, and F. Lacoste and P. Rassat for constant support.

Financial support: This study was supported by a fellowship to Sara Montailler from the Fondation de France: Fonds Inckermann.

* Address correspondence to Anna-Bella Failloux, Unité Postulante Génétique Moléculaire des Bunyaviridés, Institut Pasteur, 25–28 Rue du Docteur Roux, 75724 Paris CEDEX 15, France. E-mail: afaillou{at}pasteur.fr

Authors’ addresses: Sara Moutailler, Michèle Bouloy, and Anna-Bella Failloux, Unité Postulante Génétique Moléculaire des Bun-yaviridés, Institut Pasteur, 25–28 Rue du Dr. Roux, 75724 Paris CEDEX 15, France.






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