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Am. J. Trop. Med. Hyg., 75(6), 2006, pp. 1118-1126
Copyright © 2006 by The American Society of Tropical Medicine and Hygiene

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THE PATHOGENICITY OF MOSQUITO DENSOVIRUS (C6/36DNV) AND ITS INTERACTION WITH DENGUE VIRUS TYPE II IN AEDES ALBOPICTUS

WEI WEI, DINGYONG SHAO, XIAOJUN HUANG, JIANPING LI, HAIDONG CHEN, QINFEN ZHANG, AND JINGQIANG ZHANG*
State Key Lab for Biocontrol and Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, PR China; College of Animal Science, Zhejiang University, 310027, PR China

When Aedes albopictus larvae were infected with C6/36 densovirus (C6/36DNV), the mortality reached 97.46% within 21 days for those larvae infected at the first day after hatching, and 14.17% for control. A real-time quantitative polymerase chain reaction (qPCR) was used to trace the dynamic change of the quantity of C6/36DNV genomes in the larvae and the adults, and to study the interaction of C6/36DNV with dengue virus type II(DEN-II) in mosquitoes. It showed that C6/36DNV could persist in the adults that could transmit C6/36DNV vertically to the next generation. The quantity of C6/36DNV after DEN-II infection increased by 102~103 times in the C6/36DNV-positive mosquitoes, and the quantity of DEN-II in the C6/36DNV-positive mosquitoes was about 100 times lower than that in the C6/36DNV-negative mosquitoes, which suggested that DEN-II could remarkably stimulate the reproduction of C6/36DNV, while C6/36DNV persisted in mosquitoes could inhibit the reproduction of DEN-II. The study throws light on C6/36DNV as a possible biologic control agent against dengue virus and Ae. albopictus.

Received March 16, 2006. Accepted for publication July 20, 2006.

Acknowledgments: This work was supported by special funds of National Natural Science Foundation (No.10274106). The authors also thank Meiyu Fang for providing the Dengue virus type II.

* Address correspondence to Jingqiang Zhang, Sun Yat-Sen University, State Key Lab for Biocontrol and Institute of Entomology, Guangzhou 510275, PR China. E-mail: ww20032006{at}eyou.com

Authors’ addresses: Wei Wei, Dingyong Shao, Xiaojun Huang, Haidong Chen, Qingfen Zhang, Jingqiang Zhang, Sun Yat-Sen University, State Key Lab for Biocontrol and Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, PR China; Jianping Li, Zhejiang University College of Animal Science, Hangzhou Zhejiang 310027, PR China.




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Copyright © 2006 by the American Society of Tropical Medicine and Hygiene.