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Am. J. Trop. Med. Hyg., 73(1), 2005, pp. 204-206
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


LIMITED ADVANTAGE OF MULTIPLE CONSECUTIVE SAMPLES FOR GENOTYPING PLASMODIUM FALCIPARUM POPULATIONS DURING THE FIRST DAYS OF TREATMENT

ANNA FÄRNERT* AND ANDERS BJÖRKMAN
Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.

 

ABSTRACT

The informative value of genotyping Plasmodium falciparum populations in single blood samples was studied before and during treatment in 13 patients with P. falciparum malaria. Genotyping of the two merozoite surface proteins (msp1 [block 2] and msp2) and the glutamate-rich protein showed multiple genotypes in seven patients, and single genotypes in the remaining six patients. The same genotype profiles were detected in consecutive samples obtained every 12 hours during treatment from the respective patients, although some genotypes were cleared earlier than others. These patterns are in contrast to the extensive daily dynamics previously described in asymptomatic infections. The genotypes detected in one pre-treatment sample thus appear to reflect the parasite subpopulations of the clinical malaria infection during the following days, and additional sampling does not provide any additional information.

Received October 18, 2004. Accepted for publication December 15, 2004.

Acknowledgments: We thank the patients and staff at the Departments of Infectious Diseases and the Parasitology Laboratories at Danderyd and Huddinge University Hospitals for participating in the study. Special thanks are given to Dr. Ingela Berggren Palme, Dr. Ulf Bronner, Kerstin Engström, Marianne Lebbad, Berit Emilsson, Gunilla Herrman, and Lillemor Karlsson for their assistance during the study. We also thank Georges Snounou for most valuable support and providing the oligonucleotide primers.

Financial support: The study was supported by the Swedish International Development Agency.

* Address correspondence to Anna Färnert, Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden. E-mail: anna. farnert{at}medks.ki.se

Authors’ address: Anna Färnert and Anders Björkman, Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden, Telephone: 46-8-5177-5285, Fax: 46-8-5177-6740, E-mails: anna.farnert{at}medks.ki.se and anders.bjorkman{at}karolinska.se




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Copyright © 2005 by the American Society of Tropical Medicine and Hygiene.