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Cysticercosis, a disease caused by the larval form of Taenia solium, is diagnosed by detection of specific antibodies or by imaging techniques. Our preferred immunologic assay for cysticercosis is the enzyme-linked immunoelectrodifusion transfer blot, or immunoblot, using the lentil lectin bound antigens from larval cysts. Antibody reactivity with any one of seven glycoproteins is diagnostic for cysticercosis. To develop a simple antibody detection assay for field use, we have synthesized an 8-kD diagnostic antigen, sTs18var1 (a secreted protein with a mature size of 67 amino acids), and expressed a 50-kD membrane protein antigen, rGp50. We used these two diagnostic proteins in a quantitative Falcon assay screening test–enzyme-linked immunosorbent assay (FAST-ELISA) to measure the antibody responses in Peruvian pigs with cysticercosis. Three study designs were used. First, we followed the kinetics of antibody responses against these two diagnostic proteins in pigs with cysticercosis that were treated with oxfendazole. Second, we measured antibody response in naive experimentally infected pigs. Third, we followed the maternal antibodies against rGp50 and sTs18var1 in piglets born from sows with cysticercosis. These studies showed that antibody responses against the two diagnostic proteins in the FAST-ELISA are quantitatively correlated with infection by viable cysts, with anti-sTs18var1 activity being most responsive to the status of infection.
Received August 21, 2003. Accepted for publication April 1, 2004.
Financial support: This research was supported by International Collaborations in Infectious Disease Research–National Insitutes of Health (NIH) grant U01 AI-35894, NIH National Institute of Allergy and Infectious Diseases Tropical Medicine Research Center grant 1 P01 AI-51976-01, Wellcome grant 063109, and Bill and Melinda Gates foundation grant 23981.
Authors’ addresses: Sukwan Handali, Kathy Hancock, and Victor C. W. Tsang, Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F-13, Atlanta, GA 30341-3724, E-mails: ahi0{at}cdc.gov, kyh7{at}cdc.gov, and vct1{at}cdc.gov. Armando E. Gonzalez, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Cuadra 29 Avenida Circunvalacion s/n, San Borja, Lima, Peru, E-mail: emico{at}terra.com.pe. Hector H. Garcia, Department of Microbiology, Universidad Peruana Cayetano Heredia, Avenida Honorio Delgado 430, San Martin de Porras, Lima, Peru and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru, E-mail: hgarcia{at}terra.com.pe. Jacquelin M. Roberts, Data Management Activity, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F-22, Atlanta, GA 30341-3724, E-mail: jmr1{at}cdc.gov. Robert H. Gilman, Department of International Health, Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205, E-mail: gilmanbob{at}yahoo.com.
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  H. H. GARCIA, O. H. DEL BRUTTO, T. E. NASH, A. C. WHITE JR., V. C. W. TSANG, and R. H. GILMAN NEW CONCEPTS IN THE DIAGNOSIS AND MANAGEMENT OF NEUROCYSTICERCOSIS (TAENIA SOLIUM) Am J Trop Med Hyg, January 1, 2005; 72(1): 3 - 9. [Abstract] [Full Text] [PDF]
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