AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 69(4), 2003, pp. 450-451
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by THULLIER, P.
Right arrow Articles by CHANTEAU, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by THULLIER, P.
Right arrow Articles by CHANTEAU, S.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Plague

SHORT REPORT


SHORT REPORT: SERODIAGNOSIS OF PLAGUE IN HUMANS AND RATS USING A RAPID TEST

PHILIPPE THULLIER, VALERIE GUGLIELMO, MINO RAJERISON, AND SUZANNE CHANTEAU
Immunobiologie, Departement de Biologie des Agents Transmissibles, Centre de Recherches du Service de Sante des Armees, La Tronche, France; Institut Pasteur de Madagascar, World Health Collaborating Center for Plague, Antananarivo, Madagascar

 

ABSTRACT

Serodiagnosis of plague is very useful for its retrospective confirmation and for epidemiologic studies in humans and in rodents, since rats constitute the main natural reservoir of Yersinia pestis. We have developed a rapid test for the detection of IgG antibodies to fraction 1 (F1) based on immunochromatography and protein A to detect both human and rat IgG. When tested with reference human sera (35 positive and 37 negative), this assay showed a sensitivity of 94.3% and a specificity of 89.2%. When Rattus rattus and R. norvegicus reference sera (22 positive and 24 negative) were used, the sensitivity was 100% and the specificity was 91.7%. This simple serodiagnostic tool is of great potential value in the surveillance of plague. As far as we know, this test is the first of its kind designed for diagnosis of both humans and animals.

Received January 27, 2003. Accepted for publication July 1, 2003.

Financial support: This work was supported by a grant (PEA 803) from the Direction Générale de l’Armement.

Authors’ addresses: Philippe Thullier and Valerie Guglielmo, Immunobiologie, Département de Biologie des Agents Transmissibles, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Gresivaudan, PO Box 87, 38702 La Tronche, France, Telephone: 33-4-76-63-69-14, Fax: 33-4-76-63-69-17, E-mails: pthullier{at}yahoo.com and vguglielmo{at}crssa.net. Mino Rajerison, Institut Pasteur de Madagascar, World Health Collaborating Center for Plague, PO Box 1274, Antananarivo, Madagascar, Telephone: 261-20-22-401-64, Fax: 261-20-22-415-34, E-mail: peste{at}pasteur.mg. Suzanne Chanteau, CERMES, PO Box 10 887, Niamey, Niger, Telephone: 227-75-20-40, Fax: 227-75-31-80, E-mail: schanteau{at}cermes.ne.

Reprint requests: Philippe Thullier, Immunobiologie, Département de Biologie des Agents Transmissibles, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Gresivaudan, PO Box 87, 38702 La Tronche, France.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
C. A. Cornelius, L. E. Quenee, K. A. Overheim, F. Koster, T. L. Brasel, D. Elli, N. A. Ciletti, and O. Schneewind
Immunization with Recombinant V10 Protects Cynomolgus Macaques from Lethal Pneumonic Plague
Infect. Immun., December 1, 2008; 76(12): 5588 - 5597.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Tomaso, P. Thullier, E. Seibold, V. Guglielmo, A. Buckendahl, L. Rahalison, H. Neubauer, H. C. Scholz, and W. D. Splettstoesser
Comparison of Hand-Held Test Kits, Immunofluorescence Microscopy, Enzyme-Linked Immunosorbent Assay, and Flow Cytometric Analysis for Rapid Presumptive Identification of Yersinia pestis
J. Clin. Microbiol., October 1, 2007; 45(10): 3404 - 3407.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society of Tropical Medicine and Hygiene.