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IDENTIFICATION OF A POLYMORPHIC PLASMODIUM VIVAX MICROSATELLITE MARKER

Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite Plasmodium falciparum. Genetic markers for P. vivax would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a P. vivax telomeric clone that contains simple sequence repeats. Primers were designed to amplify this region using a two-step semi-nested polymerase chain reaction protocol. The primers did not amplify template obtained from non-infected individuals, nor DNA from primates infected with the other human malaria parasites (P. ovale, P. malariae, or P. falciparum). The marker was polymorphic in P. vivax-infected field isolates obtained from Papua New Guinea, Indonesia and Guyana. This microsatellite marker may be useful in genetic and epidemiologic studies of P. vivax malaria.

Received May 12, 2003. Accepted for publication July 14, 2003.

Acknowledgments: We thank all of the study volunteers for their willing participation. We also thank B. Keniboro and W. Kastens for fieldwork and sample acquisition, and K. Lorry for blood smear examination. Plasmodium species genomic DNA samples were kindly provided by Dr. William E. Collins (Centers for Disease Control and Prevention, Atlanta, GA).

Financial support: This work was supported by the National Institutes of Health (grant no. AI-46919).

Authors’ addresses: John C. Gomez, Department of Pathology, Case Western Reserve University, Cleveland, OH 44106-4983. David T. McNamara and Peter A. Zimmerman, The Center for Global Health and Diseases, Case Western Reserve University School of Medicine, W147D, 2109 Adelbert Road, Cleveland, OH 44106-4983, Telephone: 216-368-0508, Fax: 216-368-4825, E-mail: paz{at}po.cwru.edu. Moses J. Bockarie, Papua New Guinea Institute of Medical Research, PO Box 378, Madang, Papua New Guinea. J. Kevin Baird, Parasitic Diseases Program, U.S. Naval Medical Research Unit No. 2, American Embassy Jakarta, FPO AP 96520-8132. Jane M. Carlton, Parasite Genomics Group, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850.

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