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Am. J. Trop. Med. Hyg., 68(1), 2003, pp. 61-64
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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GENERATION AND CHARACTERIZATION OF CDNA CLONES FROM SARCOPTES SCABIEI VAR. HOMINIS FOR AN EXPRESSED SEQUENCE TAG LIBRARY: IDENTIFICATION OF HOMOLOGUES OF HOUSE DUST MITE ALLERGENS

KATJA FISCHER, DEBORAH C. HOLT, PEARLY HARUMAL, BART J. CURRIE, SHELLEY F. WALTON, AND DAVID J. KEMP
The Queensland Institute of Medical Research, The Australian Centre for International and Tropical Health and Nutrition, and The University of Queensland, Brisbane, Australia; Menzies School of Health Research, Darwin, Australia

Molecular studies on scabies, a disease of considerable human and veterinary significance, have been limited because of the difficulty of obtaining the causative organism Sarcoptes scabiei, the "itch mite." We have used skin from the bedding of crusted scabies patients as a source of mites for the construction of libraries of cDNAs from S. scabiei var. hominis in the bacteriophage {lambda} vector {lambda}ZAP express. Sequences of 145 clones established that the libraries predominantly contain sequences from S. scabiei, enabling a major sequencing program to begin. Among those sequenced to date, cDNAs encoding S. scabiei homologues of 3 house dust mite allergens—the M-177 apolipoprotein, glutathione S-transferase, and paramyosin—were identified. The availability of cDNA libraries from S. scabiei var. hominis and S. scabiei var. vulpes and the emerging public sequence databases from both opens up new possibilities in scabies research.

Received January 7, 2002. Accepted for publication June 27, 2002.

Acknowledgments: The authors thank Danny Wilson for assistance with sequencing at QIMR, and Rob Slade and Peter Wilson for assistance with sequencing at the Australian Genome Research Facility.

Financial support: This work was supported by the Australian National Health and Medical Research Council (grant 137206 and Program in Medical Genomics grant 219175).

Reprint requests: Bart J. Currie, Menzies School of Health Research, P.O. Box 41096 Casuarina, NT 0811 Australia, E-mail: bart{at}menzies.edu.au

Authors’ addresses: Katja Fischer, Deborah C. Holt, and David J. Kemp, The Queensland Institute of Medical Research, The Australian Centre for International and Tropical Health and Nutrition, and The University of Queensland, Brisbane 4029, Australia, Telephone: 61-7-33620402, Fax: 61-7-33620104. Pearly Harumal, Shelley F. Walton, and Bart J. Currie, The Menzies School of Health Research, Darwin 0811, Australia, Telephone: 61-8-89228196, Fax: 61-8-89275187.




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