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Am. J. Trop. Med. Hyg., 68(1), 2003, pp. 1-5
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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FREQUENCY OF MULTIPLE INSEMINATIONS IN FIELD-COLLECTED ANOPHELES GAMBIAE FEMALES REVEALED BY DNA ANALYSIS OF TRANSFERRED SPERM

FRÉDÉRIC TRIPET, YEYA T. TOURÉ, GUIMOGO DOLO, AND GREGORY C. LANZARO
Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Département d’ Epidémiologie des Affections Parasitaires, Ecole Nationale de Médecine et de Pharmacie, Bamako, Mali

We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry was found in six of 239 females examined, or 2.5% of the samples. Previous analyses of the molecular form of the sperm and female extracts using a PCR-based diagnostic procedure showed that two of these multiple inseminations involved cross-mating between two chromosomal/molecular forms of An. gambiae s.s. Thus polyandry occurred within-form in 1.7% of examined females while other multiple inseminations may be linked to processes of reproductive isolation between forms of An. gambiae.

Received January 14, 2002. Accepted for publication April 10, 2002.

Acknowledgments: We thank D. Elnaiem, D. Norris, and two anonymous referees for comments on the manuscript.

Financial support: The research reported here was supported by a Swiss National Science Foundation fellowship # 823A-061233 to Frédéric Tripet and NIH grant AI-40306 to Gregory C. Lanzaro.

Reprint requests: Frédéric Tripet, Dept. of Entomology, University of California Davis, Davis, CA 95616, Telephone: 530-752-5833, Fax: 530-752-1537, E-mail: ftripet{at}ucdavis.edu, frtripet{at}yahoo.com

Authors’ addresses: Frédéric Tripet and Gregory C. Lanzaro, Dept. of Entomology, University of California Davis, Davis, CA 95616. Yeya T. Touré and Guimogo Dolo, Département d’ Epidémiologie des Affections Parasitaires, Ecole Nationale de Médecine et de Pharmacie, Bamako, BP 1805, Mali.




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