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Am. J. Trop. Med. Hyg., 65(2), 2001, pp. 162-163
Copyright © 2001 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 65, Issue 2, 162-163
Copyright © 2001 by American Society of Tropical Medicine and Hygiene

Research Articles


Short report: A practical technique for the cryopreservation of Dirofilaria immitis, Brugia malayi, and Wuchereria bancrofti microfilariae

LC Bartholomay, HA Farid, E El Kordy, and BM Christensen

A technique to cryopreserve microfilariae has been developed. This method was used to cryopreserve microfilariae of Dirofilaria immitis, Brugia malayi, and Wuchereria bancrofti at a controlled rate of 1 degree C/min by use of a freezing tank. Microfilariae of each of these species retained their ability to infect susceptible mosquito species and develop to the infective stage after cryopreservation. The method presented here is quickly and easily carried out with inexpensive equipment.




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Copyright © 2001 by the American Society of Tropical Medicine and Hygiene.