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Am. J. Trop. Med. Hyg., 57(4), 1997, pp. 423-425
Copyright © 1997 by The American Society of Tropical Medicine and Hygiene

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Short Report: Leishmania DNA in Phlebotomus and Sergentomyia Species during a Kala-Azar Epidemic

Sandeep Mukherjee, Mohammed Quamarul Hassan, Anil Ghosh, Kashi Nath Ghosh, Amal Bhattacharya AND Samit Adhya
Indian Institute of Chemical Biology, Calcutta, India

The presence of Leishmania donovani DNA in sand flies caught in Indian kala-azar patients' dwellings during the epidemic of 1990–1992 was studied using the polymerase chain reaction (PCR). Amplification of miniexon-derived RNA genes and gpG3 mRNA was achieved in single Phlebotomus argentipes, P. papatasi, and Sergentomyia babu flies. The data suggest the possible involvement of multiple sandfly species in kala-azar transmission.






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Copyright © 1997 by the American Society of Tropical Medicine and Hygiene.