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Am. J. Trop. Med. Hyg., 55(3), 1996, pp. 250-253
Copyright © 1996 by The American Society of Tropical Medicine and Hygiene

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Field Detection of Sulfonamides in Urine: The Development of a New and Sensitive Test

Dwight L. Mount, Michael D. Green, Jane R. Zucker, J. Ben O. Were AND G. Daniel Todd
Entomology Branch, and Epidemiology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Clinical Research Centre, Kenya Medical Research Institute, Nairobi, Kenya

A new, field-adapted, colorimetric method for detecting sulfonamide drugs in urine is described. The method uses the color reagent, p-dimethylaminocinnamaldehyde, and has a detection limit of about 1 µg/ml. Analysis of 35 samples collected in the field, comparing results obtained with the colorimetric field test with those obtained using high-performance liquid chromatography, indicated a calculated sensitivity value of 94% and a specificity value of 94% for the test to detect the presence of sulfonamides. The field test can be modified to allow quantitation of sulfonamides in urine in field situations, using a hand-held, portable photometer for measuring the absorbance of test solutions. For this test, calculated coefficients of variation for day to day reproducibility were ≤ 5% at sulfonamide concentrations ≥ 3 µg/ml. This new test for detecting the presence of sulfonamides in urine is more sensitive and reliable than the presently used Bratton-Marshall test.






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Copyright © 1996 by the American Society of Tropical Medicine and Hygiene.