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Am. J. Trop. Med. Hyg., 49(3), 1993, pp. 335-340
Copyright © 1993 by The American Society of Tropical Medicine and Hygiene

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Cultivation of Plasmodium falciparum Parasites in a Serum-Free Medium

Ayub V. O. Ofulla, Vincent C. N. Okoye, Baldip Khan, John I. Githure, Clifford R. Roberts, Anthony J. Johnson AND Samuel K. Martin
Biomedical Sciences Research Center, Kenya Medical Research Institute, Nairobi, Kenya; Department of Hematology, Walter Reed Army Institute of Research, Washington, District of Columbia; Clinical Research Center, Kenya Medical Research Institute, Nairobi, Kenya; United States Army Medical Research Unit-Kenya, Walter Reed Army Institute of Research, Washington, District of Columbia

The elimination of serum from Plasmodium falciparum culture media could decrease costs, enhance procurement, and improve the feasibility of large-scale production of parasite material. We provide a semi-defined, serum-free formulation, of commercially available constituents that supports P. falciparum parasite growth at rates comparable with those obtained with serum-supplemented media. The medium is composed of RPMI 1640 to which HEPES, extra glucose, bicarbonate, and hypoxanthine have been added. Bovine albumin and serum-derived, lipids-cholesterol-rich mixture are then used in place of serum.




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Copyright © 1993 by the American Society of Tropical Medicine and Hygiene.