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Am. J. Trop. Med. Hyg., 33(4), 1984, pp. 560-568
Copyright © 1984 by The American Society of Tropical Medicine and Hygiene

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Different Monoclonal Antibodies against the Component 5 Specific for Trypanosoma cruzi*

O. Orozco{dagger}, D. Afchain, C. Dissous, C. Rodriguez, G. Ovlaque, J. L. Lemesre, M. Loyens AND A. Capron
Centre d'Immunologie et de Biologie Parasitaire (INSERM U 167, CNRS ERA 422), 15 rue Camille Guérin, 59019 Lille Cedex, France

Three murine monoclonal antibodies (I-35/67, II-190/30, III-160/18) produced by immunization against total epimastigote extract or component 5-enriched fractions of Trypanosoma cruzi (Tehuantepec strain) have been demonstrated to recognize the component 5 specific for T. cruzi. By immunofluorescence studies, one of these monoclonal antibodies (I-35/67) was shown to bind to the epimastigote cell surface, and the two others (II-190/30 and III-160/18) were preferentially directed against internal subcellular organelles. Immunoprecipitation using these monoclonal antibodies followed by SDS-PAGE analysis, has led to identification of four molecules with apparent molecular weights of 72 Kd, 51 Kd, 43 Kd, and 24 Kd in T. cruzi epimastigotes. Potential uses of these monoclonal antibodies in serological tests and immunochemical analysis of target antigens are discussed.

Accepted for publication December 22, 1983.


* This investigation received financial report from the Trypanosomiasis Component of the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases.

Address reprint requests to: D. Afchain, Centre d' Immunologie et de Biologie Parasitaire, Institute Pasteur, 15, rue Camille Guérin, 59019 Lille Cedex, France.


{dagger} Present address: Laboratorio de Inmunologia, Hospital La Samaritana, Bogotá, Colombia.






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Copyright © 1984 by the American Society of Tropical Medicine and Hygiene.