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No. 238, Juan Diaz (JD)

Strain: Maru 8563

Information from: Gustavo Justines, Middle America Research Unit, Box 2011, Balboa Heights, Canal Zone, 6 August 1969.

Antigenic group: Capím.

Original source: Isolated by G. Justines at Middle America Research Unit from brain of infant laboratory white mouse exposed 7 feet above ground in study area at Barriada La Concepcio in Juan Diaz, Panamá, Republic of Panamá, Lat. 9° 02' N, Long. 79° 27' W. Sentinel mouse collected 12 September 1962 after 48 hours exposure. Habitat (study area): Open coastal lowland, grass and bush vegetation. Storage: In sentinel mouse exposed in nature.

Method of isolation in laboratory: Brain from sentinel mouse showing convulsion and paralysis ground in 25% normal rabbit serum in phosphate-buffered saline pH 7.2. Inoculated 0.02 ml ic in 2-day-old mouse on 26 September 1962.

Validity of isolation: Virus reisolated.

Property of virus: Physical: No data. Chemical: RNA virus. Resistance to chemicals: Sensitive to 1:100 sodium deoxycholate; control titer 10^4.3, after-treatment titer <10^1.0.