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No. 217, Ponteves (PTV)

Strain: Larves 6

Information from: C. Hannoun, Laboratoire des Arbovirus, Institut Pasteur, Paris 15, France, 1 January 1968.

Antigenic group: Uukuniemi.

Original source: Isolated by C. Hannoun and colleagues at Paris, France, from pool of 200 Argas reflexus larvae. Adult female ticks collected by hand 18 May 1966 at Ponteves, a farm near Le Sambuc, Camargue, Rhone delta, Lat. 43° N, Long. 4° E. Habitat: Wall crevices of pigeon house on farm. Storage: Eggs laid by adult ticks in laboratory, hatched in October 1966. Larvae held alive in the laboratory for 4 weeks after hatching.

Method of isolation in laboratory: Larvae ground in 0.75% bovine albumin in Hanks's solution. Inoculated 0.02 ml ic in 2-day-old mice on 30 November 1966.

Validity of isolation: Virus reisolated.

Properties of virus: Physical: No data. Chemical: No data. Resistance to chemicals: Sensitive to 1:1,000 sodium deoxycholate; control titer 10^7.0, after-treatment titer 10^5.8. Antigenic: Hemagglutinin not produced in tests with infected suckling mouse brain extracted 3 times with fluorocarbon or with sucrose-acetone.