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Am. J. Trop. Med. Hyg., 18(5), 1969, pp. 657-661
Copyright © 1969 by The American Society of Tropical Medicine and Hygiene

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In Vitro Biosynthesis of Lipids in Blood from Ducks Infected with Plasmodium Lophurae*

William G. Brundage{dagger}, Christopher Marie Hyland, Sr.{ddagger} AND G. T. Dimopoullos
Department of Veterinary Science, Agricultural Experiment Station, Louisiana State University, Baton Rouge, Louisiana 70803

The incorporation of sodium (1-14C) acetate into the blood lipids of normal ducks and ducks infected with Plasmodium lophurae was studied. After incubation, the cells and plasma were separated, their total lipids extracted, and the lipid classes were separated by thin-layer chromatography. Individual lipid classes were examined for radioactivity by liquid-scintillation spectrometry. Activity was found in the phospholipids, sterols, free fatty acids, triglycerides, and sterol esters of both normal and infected blood cells and plasma. An additional class, monodiglycerides, was found to be active in the parasitized cells. The free fatty acids of both normal and infected plasma contained most of the activity found in their total lipids. The phospholipid fractions of normal and parasitized blood cells possessed most of the 14C activity. Parasitized blood cells demonstrated greater incorporation of 14C into their lipids than did plasma or normal blood cells.


* Supported in part by research grant AI-02250 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, U. S. Public Health Service, and Grant GY-4087 from the National Science Foundation and contract No. AT-(40-1)-3339 Modification 4 from the Atomic Energy Commission.


{dagger} Present address: Department of Biology, University of Southern Mississippi, Hattiesburg, Mississippi 39401.


{ddagger} Present address: Mount St. John Convent, Anderson Hill Road, White Plains, New York.






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Copyright © 1969 by the American Society of Tropical Medicine and Hygiene.