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Recovery of Dengue-4 Viruses from Mosquito Vectors and Patients during an Epidemic of Dengue Hemorrhagic Fever

Attempts were made to recover viruses from patients and mosquitoes during an epidemic of dengue hemorrhagic fever on an island in the Gulf of Thailand in 1967. LLC-MK₂ cell cultures were used for isolation, and identification was done by plaque-reduction neutralization tests. Aedes mosquitoes were collected resting indoors and biting outdoors. Ten strains of dengue-4 were recovered from patients' serum. Nineteen strains of dengue-4 were recovered from pools of Aedes aegypti and 10 strains from pools of Aedes albopictus. The results suggest that both species were acting as vectors of dengue-4.

^* Alternate address: SEATO Medical Project, U. S. Component, APO San Francisco 96346.