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Am. J. Trop. Med. Hyg., 18(3), 1969, pp. 440-447
Copyright © 1969 by The American Society of Tropical Medicine and Hygiene

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Isolation of Jamestown Canyon Serotype of California Encephalitis Virus from Naturally Infected Aedes Mosquitoes and Tabanids*

G. R. DeFoliart, Ralph O. Anslow, R. P. Hanson, C. D. Morris, O. Papadopoulos{dagger} AND Gladys E. Sather
Departments of Entomology and Veterinary Science, University of Wisconsin, Madison, Wisconsin, and Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania

Twelve arbovirus isolations were made from about 50,000 bloodsucking Diptera, including Culicidae (mosquitoes). Tabanidae (horseflies and deerflies), Simuliidae (black flies), and Ceratopogonidae (punkies) that were collected during 1965 in Wisconsin. The Jamestown Canyon serotype of the California virus complex was isolated from two species of Tabanidae, the horsefly, Hybomitra lasiophthalma (Macq.), and the deerfly Chrysops cincticornis (Walk.), and from the Aedes stimulans (Walk,) and the Aedes communis (DeG,) groups of mosquitoes. The virus apparently circulated among insects for only a short period (June) during 1965. Other isolations in 1965 included trivittatus virus from Aedes trivittatus and as yet unidentified arboviruses from a black fly. Simulium meridionale Riley, and a mixed pool containing mostly Culicoides.


* Work at the University of Wisconsin was supported in part by funds from NIH grant AI-00771-13. Work at the University of Pittsburgh was carried out in part under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board, and was supported by the U. S. Army Medical Research and Development Command, Department of the Army, under Contract No. DA-49-193-MD-2042; and in part by Public Health Service Research Grant No. AI-02686 from the National Institute of Allergy and Infectious Diseases. This manuscript was approved for publication by the Director of the Wisconsin Agricultural Experiment Station.


{dagger} Present address: Department of Bacteriology and Parasitology, Faculty of Veterinary Medicine, Aristotelian University, Thessaloniki, Greece.






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Copyright © 1969 by the American Society of Tropical Medicine and Hygiene.