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Influence of Vitamin E on Reproduction of Biomphalaria Glabrata Under Axenic Conditions^*,,

A semisynthetic medium that promotes growth of Biomphalaria glabrata under axenic conditions is described. This medium, when enriched with vitamin E, promotes reproduction of the mollusk. The snail was reared over three consecutive generations under germ-free conditions. The sterilization temperature of the medium is critical: when sterilized at higher temperatures than 110°C, the medium does not promote growth.

^* This work was supported by the United States Public Health Service through grant RF-24 and by Conselho Nacional de Pesquisas through grant TC-2077. Part of the permanent equipment was a gift of The Rockefeller Foundation.

Parts of this work were reported to the 16th and 18th Meetings of Sociedade Brasileira para o Progresso da Ciência, held at Ribeirão Preto (1964) and Blumenau (1966).